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Anti Gapdh Antibody, supplied by OriGene, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti gapdh  (Proteintech)
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mouse gapdh  (Proteintech)
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CLE increases AMPK phosphorylation, <t>decreases</t> <t>mTOR</t> phosphorylation at Ser2448, and enhances autophagy flux in PC12 cells. (A) The Western blot (WB) analysis of p-AMPK (Thr172), t-AMPK, p-mTOR (Ser2448), and t-mTOR protein levels in PC12 cells from the CTRL, MPP + , CLE, and MPP + +CLE groups. <t>GAPDH</t> is shown as a loading control. (B) The Quantification of p-mTOR/t-mTOR protein levels. (C) The Quantification of p-AMPK/t-AMPK protein levels. (D) The WB analysis of LC3-I and LC3-II expression. (E) The Quantification of the LC3-II/LC3-I ratio. (F) The Comparison of LC3-II/LC3-I levels in the Baf-A1, CLE, and Baf-A1+CLE groups, assessing autophagic flux. (G) The WB analysis of p62 expression. (H) The Quantification of p62 protein levels. Data are presented as mean ± SEM. Tukey’s multiple comparison test was performed after one-way ANOVA. * P < 0.05, ** P < 0.01, *** P < 0.001; ns, not significant. CLE, clemastine; CTRL, control; WB, western blot; ANOVA, analysis of variance; SEM, standard error of the mean. n = 5 independent experiments.
Mouse Gapdh, supplied by Proteintech, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse gapdh - by Bioz Stars, 2026-10
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mouse anti gapdh sc 47724 monoclonal antibodies  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology mouse anti gapdh sc 47724 monoclonal antibodies
CLE increases AMPK phosphorylation, <t>decreases</t> <t>mTOR</t> phosphorylation at Ser2448, and enhances autophagy flux in PC12 cells. (A) The Western blot (WB) analysis of p-AMPK (Thr172), t-AMPK, p-mTOR (Ser2448), and t-mTOR protein levels in PC12 cells from the CTRL, MPP + , CLE, and MPP + +CLE groups. <t>GAPDH</t> is shown as a loading control. (B) The Quantification of p-mTOR/t-mTOR protein levels. (C) The Quantification of p-AMPK/t-AMPK protein levels. (D) The WB analysis of LC3-I and LC3-II expression. (E) The Quantification of the LC3-II/LC3-I ratio. (F) The Comparison of LC3-II/LC3-I levels in the Baf-A1, CLE, and Baf-A1+CLE groups, assessing autophagic flux. (G) The WB analysis of p62 expression. (H) The Quantification of p62 protein levels. Data are presented as mean ± SEM. Tukey’s multiple comparison test was performed after one-way ANOVA. * P < 0.05, ** P < 0.01, *** P < 0.001; ns, not significant. CLE, clemastine; CTRL, control; WB, western blot; ANOVA, analysis of variance; SEM, standard error of the mean. n = 5 independent experiments.
Mouse Anti Gapdh Sc 47724 Monoclonal Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+monoclonal+antibody+for+gapdh/GAPDH+Antibody/pm42803583-215-35-44
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mouse anti gapdh sc 47724 monoclonal antibodies - by Bioz Stars, 2026-10
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mouse monoclonal anti gapdh  (Proteintech)
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Proteintech mouse monoclonal anti gapdh
CLE increases AMPK phosphorylation, <t>decreases</t> <t>mTOR</t> phosphorylation at Ser2448, and enhances autophagy flux in PC12 cells. (A) The Western blot (WB) analysis of p-AMPK (Thr172), t-AMPK, p-mTOR (Ser2448), and t-mTOR protein levels in PC12 cells from the CTRL, MPP + , CLE, and MPP + +CLE groups. <t>GAPDH</t> is shown as a loading control. (B) The Quantification of p-mTOR/t-mTOR protein levels. (C) The Quantification of p-AMPK/t-AMPK protein levels. (D) The WB analysis of LC3-I and LC3-II expression. (E) The Quantification of the LC3-II/LC3-I ratio. (F) The Comparison of LC3-II/LC3-I levels in the Baf-A1, CLE, and Baf-A1+CLE groups, assessing autophagic flux. (G) The WB analysis of p62 expression. (H) The Quantification of p62 protein levels. Data are presented as mean ± SEM. Tukey’s multiple comparison test was performed after one-way ANOVA. * P < 0.05, ** P < 0.01, *** P < 0.001; ns, not significant. CLE, clemastine; CTRL, control; WB, western blot; ANOVA, analysis of variance; SEM, standard error of the mean. n = 5 independent experiments.
Mouse Monoclonal Anti Gapdh, supplied by Proteintech, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse monoclonal anti gapdh - by Bioz Stars, 2026-10
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mouse monoclonal gapdh hrp 60008 antibodies  (Proteintech)
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Proteintech mouse monoclonal gapdh hrp 60008 antibodies
CLE increases AMPK phosphorylation, <t>decreases</t> <t>mTOR</t> phosphorylation at Ser2448, and enhances autophagy flux in PC12 cells. (A) The Western blot (WB) analysis of p-AMPK (Thr172), t-AMPK, p-mTOR (Ser2448), and t-mTOR protein levels in PC12 cells from the CTRL, MPP + , CLE, and MPP + +CLE groups. <t>GAPDH</t> is shown as a loading control. (B) The Quantification of p-mTOR/t-mTOR protein levels. (C) The Quantification of p-AMPK/t-AMPK protein levels. (D) The WB analysis of LC3-I and LC3-II expression. (E) The Quantification of the LC3-II/LC3-I ratio. (F) The Comparison of LC3-II/LC3-I levels in the Baf-A1, CLE, and Baf-A1+CLE groups, assessing autophagic flux. (G) The WB analysis of p62 expression. (H) The Quantification of p62 protein levels. Data are presented as mean ± SEM. Tukey’s multiple comparison test was performed after one-way ANOVA. * P < 0.05, ** P < 0.01, *** P < 0.001; ns, not significant. CLE, clemastine; CTRL, control; WB, western blot; ANOVA, analysis of variance; SEM, standard error of the mean. n = 5 independent experiments.
Mouse Monoclonal Gapdh Hrp 60008 Antibodies, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
mouse monoclonal gapdh hrp 60008 antibodies - by Bioz Stars, 2026-10
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anti gapdh  (OriGene)
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OriGene anti gapdh
CLE increases AMPK phosphorylation, <t>decreases</t> <t>mTOR</t> phosphorylation at Ser2448, and enhances autophagy flux in PC12 cells. (A) The Western blot (WB) analysis of p-AMPK (Thr172), t-AMPK, p-mTOR (Ser2448), and t-mTOR protein levels in PC12 cells from the CTRL, MPP + , CLE, and MPP + +CLE groups. <t>GAPDH</t> is shown as a loading control. (B) The Quantification of p-mTOR/t-mTOR protein levels. (C) The Quantification of p-AMPK/t-AMPK protein levels. (D) The WB analysis of LC3-I and LC3-II expression. (E) The Quantification of the LC3-II/LC3-I ratio. (F) The Comparison of LC3-II/LC3-I levels in the Baf-A1, CLE, and Baf-A1+CLE groups, assessing autophagic flux. (G) The WB analysis of p62 expression. (H) The Quantification of p62 protein levels. Data are presented as mean ± SEM. Tukey’s multiple comparison test was performed after one-way ANOVA. * P < 0.05, ** P < 0.01, *** P < 0.001; ns, not significant. CLE, clemastine; CTRL, control; WB, western blot; ANOVA, analysis of variance; SEM, standard error of the mean. n = 5 independent experiments.
Anti Gapdh, supplied by OriGene, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+monoclonal+antibody+for+gapdh/GAPDH+Mouse+Monoclonal+Antibody/10__1186_slash_s12974___026___04024___6-139-22-24
Average 96 stars, based on 1 article reviews
anti gapdh - by Bioz Stars, 2026-10
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Image Search Results


CLE increases AMPK phosphorylation, decreases mTOR phosphorylation at Ser2448, and enhances autophagy flux in PC12 cells. (A) The Western blot (WB) analysis of p-AMPK (Thr172), t-AMPK, p-mTOR (Ser2448), and t-mTOR protein levels in PC12 cells from the CTRL, MPP + , CLE, and MPP + +CLE groups. GAPDH is shown as a loading control. (B) The Quantification of p-mTOR/t-mTOR protein levels. (C) The Quantification of p-AMPK/t-AMPK protein levels. (D) The WB analysis of LC3-I and LC3-II expression. (E) The Quantification of the LC3-II/LC3-I ratio. (F) The Comparison of LC3-II/LC3-I levels in the Baf-A1, CLE, and Baf-A1+CLE groups, assessing autophagic flux. (G) The WB analysis of p62 expression. (H) The Quantification of p62 protein levels. Data are presented as mean ± SEM. Tukey’s multiple comparison test was performed after one-way ANOVA. * P < 0.05, ** P < 0.01, *** P < 0.001; ns, not significant. CLE, clemastine; CTRL, control; WB, western blot; ANOVA, analysis of variance; SEM, standard error of the mean. n = 5 independent experiments.

Journal: Frontiers in Aging Neuroscience

Article Title: Clemastine alleviates Parkinson’s disease pathology by activating the AMPK/mTOR pathway and enhancing autophagic flux

doi: 10.3389/fnagi.2026.1915475

Figure Lengend Snippet: CLE increases AMPK phosphorylation, decreases mTOR phosphorylation at Ser2448, and enhances autophagy flux in PC12 cells. (A) The Western blot (WB) analysis of p-AMPK (Thr172), t-AMPK, p-mTOR (Ser2448), and t-mTOR protein levels in PC12 cells from the CTRL, MPP + , CLE, and MPP + +CLE groups. GAPDH is shown as a loading control. (B) The Quantification of p-mTOR/t-mTOR protein levels. (C) The Quantification of p-AMPK/t-AMPK protein levels. (D) The WB analysis of LC3-I and LC3-II expression. (E) The Quantification of the LC3-II/LC3-I ratio. (F) The Comparison of LC3-II/LC3-I levels in the Baf-A1, CLE, and Baf-A1+CLE groups, assessing autophagic flux. (G) The WB analysis of p62 expression. (H) The Quantification of p62 protein levels. Data are presented as mean ± SEM. Tukey’s multiple comparison test was performed after one-way ANOVA. * P < 0.05, ** P < 0.01, *** P < 0.001; ns, not significant. CLE, clemastine; CTRL, control; WB, western blot; ANOVA, analysis of variance; SEM, standard error of the mean. n = 5 independent experiments.

Article Snippet: Proteins were transferred to PVDF membranes at 30 V for 90 min. Membranes were blocked with 5% non-fat milk in PBST for 1 h and incubated with primary antibodies for 2 h at room temperature: 1:50,000 mouse GAPDH (60004-1-Ig, Proteintech), 1:2,000 mouse p-mTOR (Ser2448) (67778-1-Ig, Proteintech), 1:5,000 mouse t-mTOR (66888-1-Ig, Proteintech), 1:1000 rabbit p-AMPK (Thr183/172) (ab133448, Abcam), 1:1000 mouse p62 (66184-1-Ig, Proteintech), 1:1,000 rabbit LC3-I/II (orb411847, Biorbyt), 1:5,000 mouse P62 (66184-1-Ig, Proteintech), 1:500 mouse α-syn (66412-1-Ig, Proteintech).

Techniques: Phospho-proteomics, Western Blot, Analysis, Control, Expressing, Comparison

CLE reduces α-synuclein protein levels and improves cell survival, effects inhibited by dorsomorphin (DOR). (A) Western blot (WB) and (B) The quantification of α-synuclein (α-syn) protein levels in PC12 cells from CTRL, MPP + , CLE, and MPP + +CLE groups. GAPDH is shown as a loading control. (C) Cell survival rate in CTRL, MPP + , and MPP + +CLE groups. (D, G) The WB analysis of p-AMPK (Thr172), t-AMPK, p-mTOR (Ser2448), and t-mTOR protein levels in CTRL, DOR, CLE, and DOR+CLE groups. (E, F, H) The Quantification of the indicated protein ratios. Data are presented as mean ± SEM. Tukey’s multiple comparison test was performed after one-way ANOVA. * P < 0.05, ** P < 0.01 *** P < 0.001; ns, not significant. CLE, clemastine; α-syn, α-synuclein; CTRL, control; WB, western blot; DOR, dorsomorphin; ANOVA, analysis of variance; SEM, standard error of the mean. n = 5 independent experiments.

Journal: Frontiers in Aging Neuroscience

Article Title: Clemastine alleviates Parkinson’s disease pathology by activating the AMPK/mTOR pathway and enhancing autophagic flux

doi: 10.3389/fnagi.2026.1915475

Figure Lengend Snippet: CLE reduces α-synuclein protein levels and improves cell survival, effects inhibited by dorsomorphin (DOR). (A) Western blot (WB) and (B) The quantification of α-synuclein (α-syn) protein levels in PC12 cells from CTRL, MPP + , CLE, and MPP + +CLE groups. GAPDH is shown as a loading control. (C) Cell survival rate in CTRL, MPP + , and MPP + +CLE groups. (D, G) The WB analysis of p-AMPK (Thr172), t-AMPK, p-mTOR (Ser2448), and t-mTOR protein levels in CTRL, DOR, CLE, and DOR+CLE groups. (E, F, H) The Quantification of the indicated protein ratios. Data are presented as mean ± SEM. Tukey’s multiple comparison test was performed after one-way ANOVA. * P < 0.05, ** P < 0.01 *** P < 0.001; ns, not significant. CLE, clemastine; α-syn, α-synuclein; CTRL, control; WB, western blot; DOR, dorsomorphin; ANOVA, analysis of variance; SEM, standard error of the mean. n = 5 independent experiments.

Article Snippet: Proteins were transferred to PVDF membranes at 30 V for 90 min. Membranes were blocked with 5% non-fat milk in PBST for 1 h and incubated with primary antibodies for 2 h at room temperature: 1:50,000 mouse GAPDH (60004-1-Ig, Proteintech), 1:2,000 mouse p-mTOR (Ser2448) (67778-1-Ig, Proteintech), 1:5,000 mouse t-mTOR (66888-1-Ig, Proteintech), 1:1000 rabbit p-AMPK (Thr183/172) (ab133448, Abcam), 1:1000 mouse p62 (66184-1-Ig, Proteintech), 1:1,000 rabbit LC3-I/II (orb411847, Biorbyt), 1:5,000 mouse P62 (66184-1-Ig, Proteintech), 1:500 mouse α-syn (66412-1-Ig, Proteintech).

Techniques: Western Blot, Control, Analysis, Comparison

CLE increases AMPK phosphorylation, decreases mTOR phosphorylation at Ser2448, enhances autophagy, and reduces α-synuclein levels in the mouse midbrain. (A) The WB analysis of p-AMPK (Thr172), t-AMPK, p-mTOR (Ser2448), and t-mTOR protein levels in the midbrain of mice from CTRL, MPTP, and MPTP+CLE groups. GAPDH is shown as a loading control. (B) The quantification of p-mTOR/t-mTOR levels. (C) The quantification of p-AMPK/t-AMPK levels. (D–F) The WB analysis of p62, LC3-I/II, and α-syn protein levels. (G–I) The quantification of p62, LC3-II/LC3-I ratio, and α-syn protein levels. Data are presented as mean ± SEM. Tukey’s multiple comparison test was performed after one-way ANOVA. * P < 0.05, ** P < 0.01, *** P < 0.001. CLE, clemastine; α-syn, α-synuclein; CTRL, control; WB, western blot; ANOVA, analysis of variance; SEM, standard error of the mean. n = 5 independent experiments.

Journal: Frontiers in Aging Neuroscience

Article Title: Clemastine alleviates Parkinson’s disease pathology by activating the AMPK/mTOR pathway and enhancing autophagic flux

doi: 10.3389/fnagi.2026.1915475

Figure Lengend Snippet: CLE increases AMPK phosphorylation, decreases mTOR phosphorylation at Ser2448, enhances autophagy, and reduces α-synuclein levels in the mouse midbrain. (A) The WB analysis of p-AMPK (Thr172), t-AMPK, p-mTOR (Ser2448), and t-mTOR protein levels in the midbrain of mice from CTRL, MPTP, and MPTP+CLE groups. GAPDH is shown as a loading control. (B) The quantification of p-mTOR/t-mTOR levels. (C) The quantification of p-AMPK/t-AMPK levels. (D–F) The WB analysis of p62, LC3-I/II, and α-syn protein levels. (G–I) The quantification of p62, LC3-II/LC3-I ratio, and α-syn protein levels. Data are presented as mean ± SEM. Tukey’s multiple comparison test was performed after one-way ANOVA. * P < 0.05, ** P < 0.01, *** P < 0.001. CLE, clemastine; α-syn, α-synuclein; CTRL, control; WB, western blot; ANOVA, analysis of variance; SEM, standard error of the mean. n = 5 independent experiments.

Article Snippet: Proteins were transferred to PVDF membranes at 30 V for 90 min. Membranes were blocked with 5% non-fat milk in PBST for 1 h and incubated with primary antibodies for 2 h at room temperature: 1:50,000 mouse GAPDH (60004-1-Ig, Proteintech), 1:2,000 mouse p-mTOR (Ser2448) (67778-1-Ig, Proteintech), 1:5,000 mouse t-mTOR (66888-1-Ig, Proteintech), 1:1000 rabbit p-AMPK (Thr183/172) (ab133448, Abcam), 1:1000 mouse p62 (66184-1-Ig, Proteintech), 1:1,000 rabbit LC3-I/II (orb411847, Biorbyt), 1:5,000 mouse P62 (66184-1-Ig, Proteintech), 1:500 mouse α-syn (66412-1-Ig, Proteintech).

Techniques: Phospho-proteomics, Analysis, Control, Comparison, Western Blot